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Addgene inc msn
Msn, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex4t+1/pGEX4T+moesin+1-577+(Plasmid+%2311637)/10__3390_slash_biom16020240-86-16-18
Average 91 stars, based on 2 article reviews
msn - by Bioz Stars, 2026-09
91/100 stars

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Related Articles

Clone Assay:

Article Title: A novel repressor-activator-competitor module comprising C 2 H 2 zinc finger and NAC transcription factors regulates rice grain development
Article Snippet: .. Full-length coding sequences of ZOS1-15, MED14_1, ONAC023, ONAC026, ONAC020 and MADS29 were cloned in GST (Glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and ZOS1 - 15, HDT702, OsTPL, ONAC024, ONAC023 ONAC026 and SS1/ ONAC025 CDS were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction-based cloning to produce GST_ZOS1-15, GST_MED14_1, GST_ONAC023, GST_ONAC026, GST_ONAC020, GST_MADS29, MBP_ZOS1-15, MBP_HDT702, MBP_OsTPL, MBP_LC2, MBP_ONAC024, MBP_ONAC023, MBP_ONAC026 and MBP_SS1/ ONAC025 protein expression constructs, respectively. ..

Article Title: Ermin is a p116 RIP -interacting protein promoting oligodendroglial differentiation and myelin maintenance.
Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 81771227; 91949105; 31100787; 81872303; Scientific Research Projects of Shaanxi Administration of Traditional Chinese Medicine, Grant/Award Number: 2019-ZZ-JC036 Abstract Myelin sheaths, which insulate the axons and ensure saltatory conduction of the nerve impulse, are generated and maintained via largely uncharacterized mechanisms.. Ermin is an oligodendrocyte-specific protein associated with the cytoskeleton, but how it regulates cytoskeletal remodeling during oligodendrocyte differentiation and its role in myelin maintenance are not clear.. To address this, we generated mice constitutively deficient for Ermn, the Ermin-coding gene.

Article Title: A zinc finger transcriptional repressor ZOS5-09 regulates rice grain size, starch and protein biosynthesis
Article Snippet: .. Full-length coding sequences of ZOS5-09 were cloned in GST (glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and HDA704 and OsTPL were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction based cloning to produce GST_ZOS5-09, MBP_HDA704, MBP_OsTPL protein expression constructs, respectively. ..

Binding Assay:

Article Title: A novel repressor-activator-competitor module comprising C 2 H 2 zinc finger and NAC transcription factors regulates rice grain development
Article Snippet: .. Full-length coding sequences of ZOS1-15, MED14_1, ONAC023, ONAC026, ONAC020 and MADS29 were cloned in GST (Glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and ZOS1 - 15, HDT702, OsTPL, ONAC024, ONAC023 ONAC026 and SS1/ ONAC025 CDS were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction-based cloning to produce GST_ZOS1-15, GST_MED14_1, GST_ONAC023, GST_ONAC026, GST_ONAC020, GST_MADS29, MBP_ZOS1-15, MBP_HDT702, MBP_OsTPL, MBP_LC2, MBP_ONAC024, MBP_ONAC023, MBP_ONAC026 and MBP_SS1/ ONAC025 protein expression constructs, respectively. ..

Article Title: A zinc finger transcriptional repressor ZOS5-09 regulates rice grain size, starch and protein biosynthesis
Article Snippet: .. Full-length coding sequences of ZOS5-09 were cloned in GST (glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and HDA704 and OsTPL were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction based cloning to produce GST_ZOS5-09, MBP_HDA704, MBP_OsTPL protein expression constructs, respectively. ..

Cloning:

Article Title: A novel repressor-activator-competitor module comprising C 2 H 2 zinc finger and NAC transcription factors regulates rice grain development
Article Snippet: .. Full-length coding sequences of ZOS1-15, MED14_1, ONAC023, ONAC026, ONAC020 and MADS29 were cloned in GST (Glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and ZOS1 - 15, HDT702, OsTPL, ONAC024, ONAC023 ONAC026 and SS1/ ONAC025 CDS were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction-based cloning to produce GST_ZOS1-15, GST_MED14_1, GST_ONAC023, GST_ONAC026, GST_ONAC020, GST_MADS29, MBP_ZOS1-15, MBP_HDT702, MBP_OsTPL, MBP_LC2, MBP_ONAC024, MBP_ONAC023, MBP_ONAC026 and MBP_SS1/ ONAC025 protein expression constructs, respectively. ..

Article Title: A zinc finger transcriptional repressor ZOS5-09 regulates rice grain size, starch and protein biosynthesis
Article Snippet: .. Full-length coding sequences of ZOS5-09 were cloned in GST (glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and HDA704 and OsTPL were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction based cloning to produce GST_ZOS5-09, MBP_HDA704, MBP_OsTPL protein expression constructs, respectively. ..

Expressing:

Article Title: A novel repressor-activator-competitor module comprising C 2 H 2 zinc finger and NAC transcription factors regulates rice grain development
Article Snippet: .. Full-length coding sequences of ZOS1-15, MED14_1, ONAC023, ONAC026, ONAC020 and MADS29 were cloned in GST (Glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and ZOS1 - 15, HDT702, OsTPL, ONAC024, ONAC023 ONAC026 and SS1/ ONAC025 CDS were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction-based cloning to produce GST_ZOS1-15, GST_MED14_1, GST_ONAC023, GST_ONAC026, GST_ONAC020, GST_MADS29, MBP_ZOS1-15, MBP_HDT702, MBP_OsTPL, MBP_LC2, MBP_ONAC024, MBP_ONAC023, MBP_ONAC026 and MBP_SS1/ ONAC025 protein expression constructs, respectively. ..

Article Title: Ermin is a p116 RIP -interacting protein promoting oligodendroglial differentiation and myelin maintenance.
Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 81771227; 91949105; 31100787; 81872303; Scientific Research Projects of Shaanxi Administration of Traditional Chinese Medicine, Grant/Award Number: 2019-ZZ-JC036 Abstract Myelin sheaths, which insulate the axons and ensure saltatory conduction of the nerve impulse, are generated and maintained via largely uncharacterized mechanisms.. Ermin is an oligodendrocyte-specific protein associated with the cytoskeleton, but how it regulates cytoskeletal remodeling during oligodendrocyte differentiation and its role in myelin maintenance are not clear.. To address this, we generated mice constitutively deficient for Ermn, the Ermin-coding gene.

Article Title: A zinc finger transcriptional repressor ZOS5-09 regulates rice grain size, starch and protein biosynthesis
Article Snippet: .. Full-length coding sequences of ZOS5-09 were cloned in GST (glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and HDA704 and OsTPL were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction based cloning to produce GST_ZOS5-09, MBP_HDA704, MBP_OsTPL protein expression constructs, respectively. ..

Construct:

Article Title: A novel repressor-activator-competitor module comprising C 2 H 2 zinc finger and NAC transcription factors regulates rice grain development
Article Snippet: .. Full-length coding sequences of ZOS1-15, MED14_1, ONAC023, ONAC026, ONAC020 and MADS29 were cloned in GST (Glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and ZOS1 - 15, HDT702, OsTPL, ONAC024, ONAC023 ONAC026 and SS1/ ONAC025 CDS were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction-based cloning to produce GST_ZOS1-15, GST_MED14_1, GST_ONAC023, GST_ONAC026, GST_ONAC020, GST_MADS29, MBP_ZOS1-15, MBP_HDT702, MBP_OsTPL, MBP_LC2, MBP_ONAC024, MBP_ONAC023, MBP_ONAC026 and MBP_SS1/ ONAC025 protein expression constructs, respectively. ..

Article Title: A zinc finger transcriptional repressor ZOS5-09 regulates rice grain size, starch and protein biosynthesis
Article Snippet: .. Full-length coding sequences of ZOS5-09 were cloned in GST (glutathione-S-transferase) tagged vector, pGEX4T-1 (Addgene; USA) and HDA704 and OsTPL were cloned in MBP (maltose binding protein) tagged vector, pMALc2x (Addgene; USA) by restriction based cloning to produce GST_ZOS5-09, MBP_HDA704, MBP_OsTPL protein expression constructs, respectively. ..

Article Title: The SCFβ-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis.
Article Snippet: pRK-Flag-mouse Lipin1 (Flag-Lipin1) was purchased from Addgene (32005). .. GST-Lipin1 (420 to 623 amino acids) and pBabe-Flag-Lipin1 were constructed by inserting the corresponding cDNA into pGEX4T-1 and pBabe-hygro vectors, respectively. pCMV(cytomegalovirus)– ALPP (human) was purchased from Addgene (24595) and the ALPP cDNA was subcloned into pCMV-Flag and pCMV-GST. pCMV6-FlagMyc-Lyric (human) plasmid was provided by X.Meng. ..

Article Title: Synaptotagmin-Like Protein 2a Regulates Angiogenic Lumen Formation via Weibel-Palade Body Apical Secretion of Angiopoietin-2
Article Snippet: .. The following constructs were procured for the study: pGEX4T-1 (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40059); pmCherry-C1 hSlp2-a (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40056); pEGFP-C1 hSlp2-a C2AB (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40051); pEGFP-C1 hSlp2-a del.SHD (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40050); pEGFP-C1 hSlp4-a (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40032); GFP-Rab27A (gift from William Gahl; Addgene plasmid No. 89237); pro-VWF (von Willebrand Factor)-GFP (green fluorescent protein)/mCherry (gift from Tom Carter), and pCMV6-Angpt2 (Origene MR207970). ..

Amplification:

Article Title: Paradoxical activation of c-Src as a drug-resistant mechanism.
Article Snippet: .. The human PTP-1B catalytic domain (amino acids 1-321) was amplified by PCR from pJ3H PTP-1B and subcloned into pGEX4T-1 (pJ3H PTP-1B was a gift from Ben Neel (Addgene plasmid #8601; https://www.addgene.org/8601/.org; RRID:Addgene_8601)). ..

Polymerase Chain Reaction:

Article Title: Paradoxical activation of c-Src as a drug-resistant mechanism.
Article Snippet: .. The human PTP-1B catalytic domain (amino acids 1-321) was amplified by PCR from pJ3H PTP-1B and subcloned into pGEX4T-1 (pJ3H PTP-1B was a gift from Ben Neel (Addgene plasmid #8601; https://www.addgene.org/8601/.org; RRID:Addgene_8601)). ..

Plasmid Preparation:

Article Title: Paradoxical activation of c-Src as a drug-resistant mechanism.
Article Snippet: .. The human PTP-1B catalytic domain (amino acids 1-321) was amplified by PCR from pJ3H PTP-1B and subcloned into pGEX4T-1 (pJ3H PTP-1B was a gift from Ben Neel (Addgene plasmid #8601; https://www.addgene.org/8601/.org; RRID:Addgene_8601)). ..

Article Title: The SCFβ-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis.
Article Snippet: pRK-Flag-mouse Lipin1 (Flag-Lipin1) was purchased from Addgene (32005). .. GST-Lipin1 (420 to 623 amino acids) and pBabe-Flag-Lipin1 were constructed by inserting the corresponding cDNA into pGEX4T-1 and pBabe-hygro vectors, respectively. pCMV(cytomegalovirus)– ALPP (human) was purchased from Addgene (24595) and the ALPP cDNA was subcloned into pCMV-Flag and pCMV-GST. pCMV6-FlagMyc-Lyric (human) plasmid was provided by X.Meng. ..

Article Title: Synaptotagmin-Like Protein 2a Regulates Angiogenic Lumen Formation via Weibel-Palade Body Apical Secretion of Angiopoietin-2
Article Snippet: .. The following constructs were procured for the study: pGEX4T-1 (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40059); pmCherry-C1 hSlp2-a (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40056); pEGFP-C1 hSlp2-a C2AB (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40051); pEGFP-C1 hSlp2-a del.SHD (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40050); pEGFP-C1 hSlp4-a (gift from Fernando Martin-Belmonte; Addgene plasmid No. 40032); GFP-Rab27A (gift from William Gahl; Addgene plasmid No. 89237); pro-VWF (von Willebrand Factor)-GFP (green fluorescent protein)/mCherry (gift from Tom Carter), and pCMV6-Angpt2 (Origene MR207970). ..



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